High-Density Linkage Map Constructed from a Skim Sequenced Diploid Potato Population Reveals Transmission Distortion and QTLs for Tuber Yield and Pollen Shed

Abstract The reinvention of potato, from a tetraploid clonal crop into diploid seed-based hybrid crop, requires insight in the mutational load, recombination landscape, and genetic basis fertility. Genomics-based breeding QTL discovery rely on efficient genotyping strategies such as skim sequencing, to gather genotypic information. application sequencing full-sib population non-inbred parents remains challenging. Here, we report an R implementation OutcrossSeq pipeline for diploids. We applied this large sequenced potato population. used resulting bin-markers construction high-density parent specific linkage maps, highlighting variation parental rate structural variations. subsequently explored transmission ratio distortion non-independent assortment alleles, indicative large-effect deleterious mutations. Finally, identified QTLs seedling tuber yield pots pollen shed. This study showcases range analyses, marker inference, identification distortion, map mapping, new insights that contribute potato.